DMSO: 10mM The solution is stable in the dark at −20 °C for more than 1 month if no water is present.
Appearance
powder
Color
white
Storage Condition
−20°C
ICE SUBSTRATE III - Risk and Safety
WGK Germany
3
ICE SUBSTRATE III - Introduction
N-acetyl-tyr-val-ala-asp 7-amido-4-* methylcotari (abbreviated as N-Acetyl-Tyr-Val-Asp-7-amino-4-methylcoumarin or Ac-YVAD-AMC) is a compound that belongs to the class of peptide substrates. The following is a description of the properties, uses, preparation and safety information of this compound:
Nature:
N-Acetyl-Tyr-Val-Asp-7-amino-4-methylcoumarin is a white or light yellow solid with a relative molecular mass of 607.61g/mol. It is soluble in water and soluble in organic solvents such as DMSO or methanol.
Use:
N-Acetyl-Tyr-Val-Asp-7-amino-4-methylcoumarin is often used as a substrate to detect and measure the activity of caspases. It is widely used in apoptosis studies to determine the activity of caspase-1, caspase-4 and caspase-5. It can assess the extent of apoptosis by detecting the fluorescence it produces.
Method:
The preparation method of the N-Acetyl-Tyr-Val-Asp-7-amino-4-methylcoumarin is as follows:
1. First synthesis of amide precursor: N-acetyl-tyrosyl-valyl-alanine-aspartic acid -7-amino -4-methyl coumaryl chloride (Ac-YVAD-Cl).
2. Reacting the synthesized amide precursor with aminomethyl coumarin (AMC) to generate N-Acetyl-Tyr-Val-Asp-7-amino-4-methylcoumarin (Ac-YVAD-AMC).
Safety Information:
N-Acetyl-Tyr-Val-Asp-7-amino-4-methylcoumarin has a certain toxicity to the human body, so it is necessary to pay attention to safety during use and handling. Storage should be stored in a dry, cool place, avoid direct sunlight. During operation, avoid direct contact with skin and eyes, and wear protective gloves and eye protection when necessary. Please read and understand the relevant safety data sheets and operating instructions carefully before use.